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KMID : 0359320200600020061
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Korean Journal of Veterinary Research
2020 Volume.60 No. 2 p.61 ~ p.68
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Detection and genetic analysis of zoonotic hepatitis E virus, rotavirus, and sapovirus in pigs
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Lyoo Eu-Lim
Park Byung-Joo
Ahn Hee-Seop
Han Sang-Hoon
Go Hyeon-Jeong
Kim Dong-Hwi
Lee Joong-Bok
Park Seung-Yong
Song Chang-Seon
Lee Sang-Won
Choi In-Soo
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Abstract
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The zoonotic transmission of viral diseases to humans is a serious public health concern. Pigs are frequently a major reservoir for several zoonotic viral diseases. Therefore, periodic surveillance is needed to determine the infection rates of zoonotic diseases in domestic pigs. Hepatitis E virus (HEV), rotavirus, sapovirus (SaV), and norovirus (NoV) are potential zoonotic viruses. In this study, 296 fecal samples were collected from weaned piglets and growing pigs in 13 swine farms, and the viral RNA was extracted. Partial viral genomes were amplified by reverse transcription-polymerase chain reaction (PCR) or nested-PCR using virusspecific primer sets under different PCR conditions. HEV-3, rotavirus A, and SaV genogoup 3 were detected from 11.5, 2.7, and 3.0% of the samples, respectively. On the other hand, NoV was not detected in any of the samples. Genetic analysis indicated that the nucleotide sequences of swine HEV-3 and rotavirus A detected in this study were closely related to those of human isolates. However, swine SaV was distant from the human strains. These results suggest that HEV-3 and rotavirus A can be transmitted from pigs to humans. Therefore, strict preventive measures should be implemented by workers in the swine industry to prevent infections with HEV-3 and rotavirus A excreted from pigs.
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KEYWORD
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zoonosis, hepatitis E virus, rotavirus, sapovirus, norovirus
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